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  • Reduce Contamination and Improve Sustainability

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Orion Products

  • SureSawit™ Services


High Throughput genomic services for the oil palm industry

  • Fast and simple sample collection
  • Cutting edge, automated genotyping assays
  • Easy to use customer interface for ordering and data retrieval
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In The News

  • BBC: Boosting palm oil production by mapping plant DNA +

    In this segment of BBC disrupting Agriculture learn how Orion Biosains’ oil palm screening service can boost the livelihoods of subsistence farmers in Malaysia and beyond. Read More

  • Loss of Karma transposon methylation underlies the mantled somaclonal variant of oil palm +

    The Malaysian Palm Oil Board and Orion Genomics publish in Nature the discovery of the epigenetic cause of fruit mantling, opening the way to the successful propagation of high-yielding elite oil palm lines. "Loss of Karma transposon methylation underlies the mantled somaclonal variant of oil palm,"(Ong-Abdullah et al. Nature http://dx.doi.org/10.1038/nature15365)
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  • The oil palm VIRESCENS gene controls fruit colour and encodes a R2R3-MYB +

    Oil palm, a plantation crop of major economic importance in Southeast Asia, is the predominant source of edible oil worldwide. We report the identification of the VIRESCENS (VIR) gene, which controls fruit exocarp colour and is an indicator of ripeness.
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Palm oil genome map will boost yields and protect the environment

If you are currently isolating DNA from a seedling, nursery palm or field planted palm, your current method should be compatible with the SureSawit™ SHELL Gel Kit. We have tested various home brew and commercially available DNA isolation kits and all have worked well with the kit. As with any PCR based reaction, the higher the purity of your DNA the better the reaction results. Our recommendation is the CTAB method (such as Doyle JJ, Doyle JL: A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 1987, 19:11-15. or Doyle JJ, Doyle JL: Isolation of plant DNA from fresh tissue. FOCUS 1990, 12:13-15.) or the Qiagen DNeasy Mini Plant kit (Cat No. 69104).

DNA was extracted using the CTAB method, samples A and B, or the Qiagen DNeasy Mini Plant kit (Cat No. 69104), samples C and D. dura (sample D) and tenera (samples A, B and C) fruit form were identified regardless of DNA purification method. Sample E is a no DNA control.

If you can extract DNA from your source material the kit will work. We have tested the SureSawit SHELL Gel Kit with seedlings, nursery palms of various ages and adult palms of various ages. While it can be challenging to extract DNA from very young or very old plants, with the appropriate concentration and purity of DNA the kit will work.

DNA was extracted from 3 month seedlings and new leaves on adult field planted palms. The SureSawit SHELL Gel kit was used to identify dura (D) and tenera (either D/AP or D/MP types) fruit form in the seedlings and MP and AP fruit form in the adult palms.