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Orion Products


  • SureSawit Services


High Throughput genomic services for the oil palm industry

  • Fast and simple sample collection
  • Cutting edge, automated genotyping assays
  • Easy to use customer interface for ordering and data retrieval
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In The News


  • BBC: Boosting palm oil production by mapping plant DNA +

    In this segment of BBC disrupting Agriculture learn how Orion Biosains’ oil palm screening service can boost the livelihoods of subsistence farmers in Malaysia and beyond. Read More
  • Loss of Karma transposon methylation underlies the mantled somaclonal variant of oil palm +

    The Malaysian Palm Oil Board and Orion Genomics publish in Nature the discovery of the epigenetic cause of fruit mantling, opening the way to the successful propagation of high-yielding elite oil palm lines. "Loss of Karma transposon methylation underlies the mantled somaclonal variant of oil palm,"(Ong-Abdullah et al. Nature http://dx.doi.org/10.1038/nature15365)
    Read More
  • The oil palm VIRESCENS gene controls fruit colour and encodes a R2R3-MYB +

    Oil palm, a plantation crop of major economic importance in Southeast Asia, is the predominant source of edible oil worldwide. We report the identification of the VIRESCENS (VIR) gene, which controls fruit exocarp colour and is an indicator of ripeness.
    Read More
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Palm oil genome map will boost yields and protect the environment

The components should give good results for up to 8 freeze/thaw cycles. We recommend as few freeze/thaws as possible for optimal performance. If more than 8 freeze thaw cycles are planned than split components into extra aliquots prior to refreezing.

  1. What DNA purification method are you using? If your DNA is not of sufficient purity you could see faint or missing bands of interest. Try cleaning up your DNA; we recommend the DNA Clean & Concentrator-5 (Zymo Research, Cat no. D4013 ) or use the CTAB protocol or Qiagen DNeasy kit (Cat no. 69104) for initial DNA purification. Alternatively try further diluting DNA in nuclease free water. Crude DNA extractions can contain a greater concentration of PCR inhibitors. Diluting low purity DNA even below 1 ng/uL will intensify faint or missing bands of interest.
  2. Letting the PCR reaction sit for an extended period of time in a warm (>23 °C) environment may reduce band of interest, while increasing non-specific amplification (smear and primer dimer amplification). If this occurs, keeping reactions cold during set up will intensify the bands of interest.
  3. Some DNA elution buffers can contain high levels of EDTA (DNeasy kit buffer AE has an EDTA concentration of 0.5 mM), which can chelate the Mg+2 ions needed by the Taq Polymerase for PCR. We recommend further diluting eluted DNA in Nuclease-free water (2-5 fold dilutions) or Low EDTA (0.1 mM) TE Buffer (>5 fold dilutions).
  4. Numerous freeze thaws on the primers and master mix can decrease the intensity of the band of interest and increase non-specific PCR (smearing and primer dimers).